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tl;dr
*You play as the first Big Daddy ever, complete with upgradable Rivet Gun and Drill.
*Plasmids are more customizable now, and you can combine them. Upgrading plasmids, instead of making them stronger, will give them new powers, such as adding a flamethrower esque ability to the incinerate plasmid. When you combine plasmids, you can do stuff like using the cyclone plasmid, then burn it with the incinerate plasmid to create a fire-tornado.
*The level designer for Fort Frolic returns to design the entire game this time, should make for an awesome experience.
*Throughout the entire game the Big Sister persues you, and is quicker, stronger, and scarier than any Big Daddy.
*You can choose to either harvest or adopt Little Sisters in Sea of Dreams. Harvesting will, like BioShock original, get your more Adam but at the expense of the Little Sister's life. Adopting will allow a Little Sister to follow you around Rapture in search of dead bodies to get Adam from, which you can use. During these Adam extract moments you will have to fend off Splicers like during the last section of BioShock when you were a Big Daddy. Also, choosing whether to harvest or adopt Little Sisters will have more of an impact on Rapture itself and the story than it did in the original game.
*You can explore underwater areas of Rapture and leer into the windows of it, viewing areas seen in the past game as well as areas you may see as your progress through the game. They think of these areas as sort of "breathers" from the action, as there are no splicers to be worried about confronting or Big Sister.
*There will be multiplayer this time around, but they state that despite this single player will still be the main focus of the game.
Everything sounds great so far, I'm not wild about the inclusion of multiplayer, but if they have it so a separate team does multiplayer while the main team focuses on single player it should be fine. I'm happy to hear that the radios will return to tell us more of the story Rapture has to offer, and playing as a Big Daddy sounds great. I can't wait, this should be great.