Ants in my pants while in Deset Temple

Do you need the all purpose bug killer to kill these?

jk

What do you do to kill them? Do they die like the orange balls in wind waker (the ants behave similary)

Are you talking about the twilight bugs that cling on to you in Arbiter's Grounds and make you slow walk?

If so, change into a wolf, turn on your senses, and do a spin attack. They will fall right off. Now that I think about it, they are a bit like the orange balls in WW.

You just have to do a spin attack. Doesn't have to be in Wolf form.

Keep your latern out, as long as it's burning they'll stay away from you.